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http://hdl.handle.net/10662/14532
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Campo DC | Valor | idioma |
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dc.contributor.author | Gaitskell Phillips, Gemma | - |
dc.contributor.author | Martín Cano, Francisco Eduardo | - |
dc.contributor.author | Ortiz Rodríguez, José Manuel | - |
dc.contributor.author | Silva Rodríguez, Antonio | - |
dc.contributor.author | Gil Anaya, María Cruz | - |
dc.contributor.author | Ortega Ferrusola, Cristina | - |
dc.contributor.author | Peña Vega, Fernando Juan | - |
dc.date.accessioned | 2022-04-22T07:21:01Z | - |
dc.date.available | 2022-04-22T07:21:01Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.uri | http://hdl.handle.net/10662/14532 | - |
dc.description.abstract | Although cryopreservation is widely used in animal breeding, the technique is still suboptimal. The population of spermatozoa surviving the procedure experiences changes attributed to alteration in their redox regulation. In order to expand our knowledge regarding this particular aspect, the proteome in fresh and frozen thawed aliquots of equine spermatozoa was studied to identify the proteins most severely affected by the procedure. If alteration of redox regulation is a major factor explaining cryodamage, proteins participating in redox regulation should be principally affected. Using a split sample design, 30 ejaculates from 10 different stallions were analyzed as fresh spermatozoa, and another aliquot from the same ejaculate was analyzed as a frozen thawed sample. The proteome was studied under both conditions using UHPLC-MS/MS and bioinformatic analysis conducted to identify discriminant variables between both conditions. Data are available through the ProteomeXchange Consortium with identifier PXD022236. The proteins most significantly reduced were Aldo-keto reductase family 1 member B (p = 2.2 × 10–17) and Superoxide dismutase (Cu–Zn) (p = 4.7 × 10–14). This is the first time that SOD1 has been identified as a discriminating variable using bioinformatic analysis, where it was one of the most highly significantly different proteins seen between fresh and frozen thawed semen. This finding strongly supports the theory that alteration in redox regulation and oxidative stress is a major factor involved in cryodamage and suggests that control of redox regulation should be a major target to improve current cryopreservation procedures. | es_ES |
dc.description.sponsorship | The authors received financial support for this study from the Ministry of Science-FEDER, Madrid, Spain, through grants AGL2017-83149-R and PID2019-107797RA-I00 AEI/10.13039/501100011033, Junta de Extremadura-FEDER (GR 18008 and IB16030). JMOR holds a PhD grant from the Junta de Extremadura- FEDER (PD 18005), Mérida, Spain. GG-P holds a PhD grant from the Ministry of Science, Madrid, Spain (PRE2018-083354). | es_ES |
dc.format.extent | 12 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | ACS (American Chemical Society) | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Spermatozoa | es_ES |
dc.subject | Equids | es_ES |
dc.subject | Cryopreservation | es_ES |
dc.subject | Redox | es_ES |
dc.subject | Proteomics | es_ES |
dc.subject | Espermatozoides | es_ES |
dc.subject | Criopreservación | es_ES |
dc.subject | Équidos | es_ES |
dc.subject | Reacciones redox | es_ES |
dc.subject | Péptidos y proteinas | es_ES |
dc.title | In Stallion Spermatozoa, Superoxide Dismutase (Cu–Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | openAccess | es_ES |
dc.subject.unesco | 3104.05 Équidos | es_ES |
dc.subject.unesco | 3104.11 Reproducción | es_ES |
dc.subject.unesco | 3303.13 Tecnología de la Conservación | es_ES |
dc.subject.unesco | 2401.04 Citología Animal | es_ES |
dc.subject.unesco | 3302 Tecnología Bioquímica | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Gaitskell-Phillips, G.,Martín-Cano, F.E.,Ortiz-Rodríguez, J.M.,Silva-Rodríguez, A.,Gil, M.C.,Ortega-Ferrusola, M.C. & Peña, F.J. (2021). In stallion spermatozoa, superoxide dismutase (Cu–Zn) (SOD1) and the aldo-keto-reductase family 1 member b (AKR1B1) are the proteins most significantly reduced by cryopreservation. Journal of Proteome Research, 20 (5), 2435-2446 DOI: 10.1021/acs.jproteome.0c00932 | es_ES |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Medicina Animal | es_ES |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acs.jproteome.0c00932 | es_ES |
dc.identifier.doi | 10.1021/acs.jproteome.0c00932 | - |
dc.identifier.publicationtitle | Journal of Proteome Research | es_ES |
dc.identifier.publicationissue | 5 | es_ES |
dc.identifier.publicationfirstpage | 2435 | es_ES |
dc.identifier.publicationlastpage | 2446 | es_ES |
dc.identifier.publicationvolume | 20 | es_ES |
dc.identifier.e-issn | 1535-3907 | - |
dc.identifier.orcid | 0000-0002-3248-6493 | - |
Colección: | DMANI - Artículos |
Archivos
Archivo | Descripción | Tamaño | Formato | |
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acs.jproteome.0c00932.pdf | 2,05 MB | Adobe PDF | Descargar |
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