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http://hdl.handle.net/10662/19904
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Campo DC | Valor | idioma |
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dc.contributor.author | Iniesta, Virginia | - |
dc.contributor.author | Belinchón Lorenzo, Silvia | - |
dc.contributor.author | Soto, Manuel | - |
dc.contributor.author | Fernández Cotrina, Javier | - |
dc.contributor.author | Muñoz Madrid, Rubén | - |
dc.contributor.author | Monroy, Isabel | - |
dc.contributor.author | Baz, Victoria | - |
dc.contributor.author | Gómez Luque, Adela | - |
dc.contributor.author | Parejo, Juan Carlos | - |
dc.contributor.author | Alonso, Carlos | - |
dc.contributor.author | Gómez Nieto, Luis Carlos | - |
dc.date.accessioned | 2024-02-05T11:42:54Z | - |
dc.date.available | 2024-02-05T11:42:54Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0001-706X | - |
dc.identifier.uri | http://hdl.handle.net/10662/19904 | - |
dc.description.abstract | Hair can accumulate foreign chemical or biological substances. Recently, it has been reported that parasite DNA can also be detected in the hair of Leishmania infantum infected dogs. The aim of this work has been to find out whether parasite DNA incorporates in the hair of Leishmania major experimentally infected animals. For this purpose, a group of 4 BALB/c mice, intradermally inoculated in both ears with 1000 L. major V1 strain promastigote forms, was monitored for parameters associated to the infection during 35 days. Weekly, ear swelling was measured, and hair samples from ears and leg were collected. Blood samples were obtained before challenge and at day 35 post infection, when parasite load was measured in ear, lymph node and spleen by limit dilution. Ear swelling and other parameters observed in the infected mice were consistent with those described for this model. The presence of parasite kinetoplast DNA (kDNA) was detected by Real Time PCR in all ear and leg hair samples at the final timepoint. These data suggests that hair is a specialized tissue in the sequestration and removal of foreign DNA. Detection of DNA in hair could be, therefore, a useful tool to chronologically record the infection process during experimental mice assays. | es_ES |
dc.format.extent | 5 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | ELSEVIER SIENCE BV | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hair | es_ES |
dc.subject | Keratinocytes | es_ES |
dc.subject | Kinetoplast DNA (kDNA) | es_ES |
dc.subject | Leishmania major | es_ES |
dc.subject | Mice | es_ES |
dc.subject | Real time PCR | es_ES |
dc.subject | Pelaje | - |
dc.subject | Queratinocitos | - |
dc.subject | ADN del cinetoplasto (ADNk) | - |
dc.subject | Leishmania mayor | - |
dc.subject | Ratones | - |
dc.subject | PCR en tiempo real | - |
dc.title | Detection and chronology of parasitic kinetoplast DNA presence in hair of experimental Leishmania major infected BALB/c mice by Real Time PCR | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | closedAccess | es_ES |
dc.subject.unesco | 2401.12 Parasitología Animal | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Virginia Iniesta, Silvia Belinchón-Lorenzo, Manuel Soto, Javier Fernández-Cotrina, Rubén Muñoz-Madrid, Isabel Monroy, Victoria Baz, Adela Gómez-Luque, Juan Carlos Parejo, Carlos Alonso, Luis Carlos Gómez Nieto, Detection and chronology of parasitic kinetoplast DNA presence in hair of experimental Leishmania major infected BALB/c mice by Real Time PCR, Acta Tropica, Volume 128, Issue 3, 2013, Pages 468-472, ISSN 0001-706X, https://doi.org/10.1016/j.actatropica.2013.07.007. | - |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Enfermería | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Sanidad Animal | - |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Medicina Animal | - |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0001706X13001873?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.actatropica.2013.07.007 | - |
dc.identifier.publicationtitle | Acta Topica | es_ES |
dc.identifier.publicationissue | 3 | es_ES |
dc.identifier.publicationfirstpage | 468 | es_ES |
dc.identifier.publicationlastpage | 472 | es_ES |
dc.identifier.publicationvolume | 128 | es_ES |
dc.identifier.orcid | 0000-0003-0228-4767 | es_ES |
dc.identifier.orcid | 0000-0003-3713-0047 | - |
dc.identifier.orcid | 0000-0002-7244-9005 | - |
Colección: | DENFE - Artículos |
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