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http://hdl.handle.net/10662/19914
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Campo DC | Valor | idioma |
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dc.contributor.author | Belinchón Lorenzo, Silvia | - |
dc.contributor.author | Parejo Rosas, Juan Carlos | - |
dc.contributor.author | Iniesta, Virginia | - |
dc.contributor.author | Fernández Cotrina, Javier | - |
dc.contributor.author | Muñoz Madrid, Rubén | - |
dc.contributor.author | Monroy, Isabel | - |
dc.contributor.author | Baz, Victoria | - |
dc.contributor.author | Gómez Luque, Adela | - |
dc.contributor.author | Serrano Aguilera, Francisco Javier | - |
dc.contributor.author | Barneto, José Luis | - |
dc.contributor.author | Gómez Nieto, Luis Carlos | - |
dc.date.accessioned | 2024-02-05T11:58:15Z | - |
dc.date.available | 2024-02-05T11:58:15Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0304-4017 | - |
dc.identifier.uri | http://hdl.handle.net/10662/19914 | - |
dc.description.abstract | Nowadays, searching for alternative non-invasive methods for molecular diagnosis of canine visceral leishmaniosis is getting increasingly important. We previously described the presence of Leishmania kinetoplast DNA (kDNA) in canine hair; in this case we hypothesized whether foreign DNA might be present in cerumen of dogs with leishmaniosis, and be detected by Real time quantitative PCR (qPCR). A population of 38 dogs that lived in Leishmania endemic areas was divided in two groups: A (33 dogs with confirmed leishmaniosis by serological techniques) and B (5 healthy dogs). Blood, lymph node, bone marrow and cerumen samples from all animals were tested for the presence of parasite kDNA. Our method was 100% specific, and in dogs from group A, Leishmania infantum kDNA was detected and quantified in the 100% of lymph node samples, in 90.9% of cerumen samples, in 88.5% of the bone marrow samples and in 57.6% of the blood samples. The qPCR-cerumen is a new non-invasive method that shows a high potential for the diagnosis of zoonotic visceral leishmaniosis. | es_ES |
dc.description.sponsorship | This investigation was partially supported by grants from the research agreement SGTRI 112/06 between LETI Laboratories S.L.U. and University of Extremadura (Spain). | - |
dc.format.extent | 4 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | ELSEVIER SIENCE BV | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cerumen | es_ES |
dc.subject | Dog | es_ES |
dc.subject | Kinetoplast DNA (kDNA) | es_ES |
dc.subject | Leishmania infantum | es_ES |
dc.subject | Real Time PCR | es_ES |
dc.subject | Perro | - |
dc.subject | ADN del cinetoplasto (ADNk) | - |
dc.subject | Leishmania infantil | - |
dc.subject | PCR en tiempo real | - |
dc.title | First detection of Leishmania kDNA in canine cerumen samples by qPCR | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | closedAccess | es_ES |
dc.subject.unesco | 2401.12 Parasitología Animal | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Silvia Belinchón-Lorenzo, Juan Carlos Parejo, Virginia Iniesta, Javier Fernández-Cotrina, Rubén Muñoz-Madrid, Isabel Monroy, Victoria Baz, Adela Gómez-Luque, Francisco Javier Serrano-Aguilera, José Luis Barneto, Luis Carlos Gómez-Nieto, First detection of Leishmania kDNA in canine cerumen samples by qPCR, Veterinary Parasitology, Volume 228, 2016, Pages 65-68, ISSN 0304-4017, https://doi.org/10.1016/j.vetpar.2016.05.021 | - |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Enfermería | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Medicina Animal | - |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Sanidad Animal | - |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0304401716301868 | es_ES |
dc.identifier.doi | 10.1016/j.vetpar.2016.05.021 | - |
dc.identifier.publicationtitle | Veterinary Parasitology | es_ES |
dc.identifier.publicationfirstpage | 65 | es_ES |
dc.identifier.publicationlastpage | 68 | es_ES |
dc.identifier.publicationvolume | 228 | es_ES |
dc.identifier.orcid | 0000-0003-0228-4767 | es_ES |
dc.identifier.orcid | 0000-0003-3713-0047 | - |
dc.identifier.orcid | 0000-0002-7244-9005 | - |
Colección: | DENFE - Artículos |
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Archivo | Descripción | Tamaño | Formato | |
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