Expanded equine cumulus-oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus-oocyte complexes

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Expanded equine cumulus-oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus-oocyte complexes

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dc.contributor.author González Fernández, Lauro
dc.contributor.author Sánchez-Calabuig, M.J.
dc.contributor.author Alves, M.G.
dc.contributor.author Oliveira, P.F.
dc.contributor.author Macedo, S.
dc.contributor.author Gutiérrez-Adán, A.
dc.contributor.author Rocha, A.
dc.contributor.author Macías García, Beatriz
dc.date.accessioned 2018-01-18T11:24:16Z
dc.date.available 2018-01-18T11:24:16Z
dc.date.issued 2018-01-18
dc.identifier.uri http://hdl.handle.net/10662/6856
dc.description Publicado en: Reproduction, Fertility and Development 30(2) 297-306 https://doi.org/10.1071/RD16441 Submitted: 4 November 2016 Accepted: 6 June 2017 Published: 6 July 2017 es_ES
dc.description.abstract Equine cumulus-oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This divergence could be related to different glucose metabolism. To test this hypothesis eCOCs, cCOCs, and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30 hours and the maturation rate, glucose metabolism, and expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. Significant differences were found between eCOCs and cCOCs maturation rates (50% vs. 21.7 %; n = 192 and 46 respectively, p < 0.001), glucose consumption (1.8 ± 0.5 vs. 27.9 ± 5.9 nmol/COC; mean ± SEM), pyruvate production (0.1 ± 0.0 vs. 2.4 ± 0.8 nmol/COC; mean ± SEM) and lactate production (4.7 ± 1.3 vs. 64.1 ± 20.6 nmol/COC; mean ± SEM) respectively (p < 0.05). Moreover, similar glucose consumption was observed for EC and CC. Hyaluronan binding protein (TNFAIP6) expression was increased in eCOCs and EC, solute carrier family 2 (facilitated glucose transporter) member 1 (SLC2A1) was increased in eCOCs, while glycolysis-related enzymes and solute carrier family 2 (facilitated glucose transporter) member 3 (SLC2A3) expression did not vary between COCs or mural granulosa cell type. Our data demonstrate that metabolic and genomic differences exist between eCOCs and cCOCs and mural granulosa cells in the horse. es_ES
dc.description.sponsorship This work was financed by AGL2015-66145-R funding from the Spanish Ministry of Economy, Industry and 364 Competitiveness and by AGL2015-73249-JIN(AEI/FEDER/UE) from the "Agencia Estatal de Investigación" 365 (AEI) (Spanish Ministry of Economy, Industry and Competitiveness) and "Fondo Europeo de Desarrollo 366 Regional" (FEDER). Beatriz Macías-García holds a postdoctoral grant "Juan de la Cierva Incorporación"(IJCI-367 2014-19428) from the Spanish Ministry of Economy, Industry and Competitiveness. L.G.-F. (Grant reference: 368 SFRH/BPD/85532/2012) and B. M.-G. (Grant reference: SFRH/BPD/84354/2012) were also partially funded by 369 Fundação para a Ciência e a Tecnologia (Portuguese Ministry for Science, Technology and Higher Education) co370 funded by Programa Operacional Potencial Humano (POPH) financed by European Social Fund (ESF) and 371 Portuguese national funds from Ministry for Science, Technology and Higher Education. The authors thank 372 CECA/ICETA (University of Porto) for funding the abattoir dislocations. The collaboration of Linda Rosa 373 Abattoir is highly appreciated. The authors wish to thank the Laboratory of Applied Physiology (Department of 374 Aquatic Production) of the ICBAS (University of Porto) and especially Mariana Hinzmann for allowing us to use 375 their fluorescence microscope. RNAlater was kindly provided by Dr. Michael Jowers. es_ES
dc.format.extent 25 p. es_ES
dc.format.mimetype application/pdf en_US
dc.language.iso eng es_ES
dc.rights Atribución-NoComercial-SinDerivadas 3.0 España *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/ *
dc.subject Maduración in vitro es_ES
dc.subject Glicólisis es_ES
dc.subject Resonancia magnética nuclear es_ES
dc.subject Caballo es_ES
dc.subject In vitro maturation es_ES
dc.subject Glycolysis es_ES
dc.subject Nuclear magnetic resonance es_ES
dc.subject Horse es_ES
dc.title Expanded equine cumulus-oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus-oocyte complexes es_ES
dc.type article es_ES
europeana.type TEXT en_US
dc.rights.accessRights openAccess es_ES
dc.subject.unesco 3104.11 Reproducción es_ES
europeana.dataProvider Universidad de Extremadura. España es_ES
dc.type.version submittedVersion es_ES
dc.contributor.affiliation Universidad de Extremadura. Grupo de investigación "Señalización Intracelular y Tecnología de la Reproducción" (SINTREP) es_ES
dc.contributor.affiliation Universidad de Extremadura. Departamento de Fisiología
dc.contributor.affiliation Universidade do Porto. Portugal
dc.contributor.affiliation Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
dc.contributor.affiliation Centro de Cirugía de Mínima Invasión Jesús Usón. Cáceres
dc.relation.publisherversion http://www.publish.csiro.au/rd/RD16441 es_ES
dc.relation.publisherversion https://doi.org/10.1071/RD16441 es_ES
dc.identifier.doi 10.1071/RD16441
dc.identifier.publicationtitle Reproduction, Fertility and Development es_ES
dc.identifier.publicationissue 2 es_ES
dc.identifier.publicationfirstpage 297 es_ES
dc.identifier.publicationlastpage 306 es_ES
dc.identifier.publicationvolume 30 es_ES

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