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dc.contributor.authorCarvajal González, José María-
dc.contributor.authorMulero Navarro, Sonia María-
dc.contributor.authorSmith, Michael-
dc.contributor.authorMlodzik, Marek-
dc.date.accessioned2018-01-19T13:26:46Z-
dc.date.available2018-01-19T13:26:46Z-
dc.date.issued2016-
dc.identifier.issn2160-1836-
dc.identifier.urihttp://hdl.handle.net/10662/6891-
dc.description.abstractMost mutant alleles in the Fz-PCP pathway genes were discovered in classic Drosophila screens looking for recessive loss-of-function (LOF) mutations. Nonetheless, although Fz-PCP signaling is sensitive to increased doses of PCP gene products, not many screens have been performed in the wing under genetically engineered Fz overexpression conditions, mostly because the Fz phenotypes were strong and/or not easy to score and quantify. Here, we present a screen based on an unexpected mild Frizzled gain-of-function (GOF) phenotype. The leakiness of a chimeric Frizzled protein designed to be accumulated in the endoplasmic reticulum (ER) generated a reproducible Frizzled GOF phenotype in Drosophila wings. Using this genotype, we first screened a genome-wide collection of large deficiencies and found 16 strongly interacting genomic regions. Next, we narrowed down seven of those regions to finally test 116 candidate genes. We were, thus, able to identify eight new loci with a potential function in the PCP context. We further analyzed and confirmed krasavietz and its interactor short-stop as new genes acting during planar cell polarity establishment with a function related to actin and microtubule dynamics.es_ES
dc.description.sponsorshipTrabajo financiado por: Institute and General Medical Sciences and National Eye National Institutes of Health. Beca para Marek Mlodzik Ministerio de Economía y Competitividad. Beca BFU2014-54699-P, para José María Carvajal González Junta de Extremadura. Ayuda para la Atracción y Retención del Talento Invetigador, para José María Carvajal Gonzálezes_ES
dc.format.extent11 p.es_ES
dc.format.mimetypeapplication/pdfen_US
dc.language.isoenges_ES
dc.publisherGenetics Society of Americaes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectPolaridad celular planares_ES
dc.subjectDrosophilaes_ES
dc.subjectFrizzledes_ES
dc.subjectKrasavietzes_ES
dc.subjectParada cortaes_ES
dc.subjectPlanar cell polarityes_ES
dc.subjectShort-stopes_ES
dc.titleA novel frizzled-based screening tool identifies genetic modifiers of planar cell polarity in drosophila wingses_ES
dc.typearticlees_ES
dc.description.versionpeerReviewedes_ES
europeana.typeTEXTen_US
dc.rights.accessRightsopenAccesses_ES
dc.subject.unesco2302.27 Proteínases_ES
europeana.dataProviderUniversidad de Extremadura. Españaes_ES
dc.identifier.bibliographicCitationCarvajal González, J. M.; Mulero Navarro, S.; Smith, M. y Mlodzik, M. (2016). A novel frizzled-based screening tool identifies genetic modifiers of planar cell polarity in drosophila wings. G3: genes, genomas, genetics, 6, 12, 3963-6973. ESSN 2160-1836es_ES
dc.type.versionpublishedVersiones_ES
dc.contributor.affiliationIcahn School of Medicine at Mount Sinai. USAes_ES
dc.contributor.affiliationUniversidad de Extremadura. Departamento de Bioquímica, Biología Molecular y Genéticaes_ES
dc.relation.publisherversionhttp://www.g3journal.org/content/6/12/3963es_ES
dc.identifier.doi10.1534/g3.116.035535-
dc.identifier.publicationtitleG3: genes, genomas, geneticses_ES
dc.identifier.publicationissue12es_ES
dc.identifier.publicationfirstpage3963es_ES
dc.identifier.publicationlastpage3973es_ES
dc.identifier.publicationvolume6es_ES
Colección:DBYBM - Artículos

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