Please use this identifier to cite or link to this item: http://hdl.handle.net/10662/7565
Title: AMP-activated kinase in human spermatozoa: Identification, intracellular localization, and key function in the regulation of sperm motility
Authors: Calle Guisado, Violeta
Hurtado de Llera, Ana
Martín Hidalgo, David
Mijares Gordún, José
Gil Anaya, María Cruz
Álvarez Miguel, Ignacio Santiago
Bragado González, María Julia
García Marín, Luis Jesús
Centro de Cirugía de Mínima Invasión Jesús Usón. Cáceres
Universidad de Extremadura. Departamento de Anatomía, Biología Celular y Zoología
Universidad de Extremadura. Departamento de Bioquímica, Biología Molecular y Genética
Universidad de Extremadura. Departamento de Fisiología
Universidad de Extremadura. Departamento de Medicina Animal
Keywords: AMP-quinasa activada
Espermatozoides humanos
Inmunolocalización
Motilidad de los espermatozoides
Calidad del esperma
AMP‑activated kinase
Human spermatozoa
Immunolocalization
Sperm motility
Sperm quality
Issue Date: 2017
Publisher: Medknow
Abstract: AMP‑activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work’s aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho‑Thr172‑AMPK were analyzed by Western blotting and indirect immunofluorescence. High‑ and low‑quality sperm populations were separated by a 40%–80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System (ISAS) in the presence or absence of the AMPK inhibitor compound C (CC). AMPK is localized along the human spermatozoa, at the entire acrosome, midpiece and tail with variable intensity, whereas its active form, phospho‑Thr172‑AMPK, shows a prominent staining at the acrosome and sperm tail with a weaker staining in the midpiece and the postacrosomal region. Interestingly, spermatozoa bearing an excess residual cytoplasm show strong AMPK staining in this subcellular compartment. Both AMPK and phospho‑Thr172‑AMPK human spermatozoa contents exhibit important individual variations. Moreover, active AMPK is predominant in the high motility sperm population, where shows a stronger intensity compared with the low motility sperm population. Inhibition of AMPK activity in human spermatozoa by CC treatment leads to a significant reduction in any sperm motility parameter analyzed: percent of motile sperm, sperm velocities, progressivity, and other motility coefficients. This work identifies and points out AMPK as a new molecular mechanism involved in human spermatozoa motility. Further AMPK implications in the clinical efficiency of assisted reproduction and in other reproductive areas need to be studied.
URI: http://hdl.handle.net/10662/7565
ISSN: 1008-682X
Appears in Collections:DABCZ - Artículos

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