Phenolic profiles and antioxidant properties of Physalis angulata L. as quality indicators

DSpace/Manakin Repository

español português english

Phenolic profiles and antioxidant properties of Physalis angulata L. as quality indicators

Show full item record

Title: Phenolic profiles and antioxidant properties of Physalis angulata L. as quality indicators
Author: Cobaleda Velasco, Marcos; Alanis Bañuelos, Ruth Elizabeth; Almaraz Abarca, Norma; Rojas López, Marlon; González Valdéz, Laura Silvia; Ávila Reyes, José Antonio; Morales Rodrigo, Sara
Abstract: ANTECEDENTES: Reportes previos han informado sobre la especificidad de los perfiles fenólicos de las plantas y sobre la asociación entre éstos y las propiedades antioxidantes. La especificidad de los perfiles fenólicos permitiría desarrollar marcadores químicos de calidad para plantas medicinales. Sin embargo, la composición fenólica puede cambiar durante el crecimiento. Para Physalis angulata se desconoce la variación asociada al desarrollo de la composición fenólica y de las propiedades antioxidantes. OBJETIVOS: Determinar la composición fenólica y propiedades antioxidantes de diferentes partes de P. angulata en cuatro estados de desarrollo y evaluar su potencial como indicadores químicos de calidad. MÉTODOS: Los perfiles fenólicos se determinaron por HPLC-DAD, las propiedades antioxidantes por métodos espectrométricos, y la discriminación entre muestras por un análisis de agrupamiento. RESULTADOS: Se encontraron 21 compuestos fenólicos, los cuales se acumularon de manera específica por tejido y edad de la planta. No se observó una clara diferencia entre los contenidos fenólicos y las propiedades antioxidantes de las muestras. Las flores maduras sobresalieron por su contenido fenólico (6,50 mg/g de tejido fresco) y capacidad antioxidante total (416,1 μg/mL). Las hojas jóvenes por su contenido de flavonoides (37,86 μg/g tejido fresco) y poder reductor (A700nm = 4,04). Los cálices inmaduros por su concentración de ácidos fenólicos (1,28 μg/g de tejido fresco) y actividad bloqueadora de radicales libres (EC50 = 0,12 mg/mL). CONCLUSIONES: Los perfiles fenólicos representan fiables marcadores químicos de calidad para determinar la edad de la planta, el origen tisular, la autenticidad y la adulteración de preparaciones herbales de P. angulata.CONTEXT: Previous reports informed about the species-specific condition of plant phenolic profiles and the association between phenolic composition and plant antioxidant properties. The species-specific condition of phenolic patterns offers the opportunity to develop chemical quality markers for plants used in traditional medicine. However, changes in phenolic composition can occur during plant growth. Physalis angulata is an important medicinal plant for which the age-related variation of phenolic composition and antioxidant properties is unknown. AIMS: To assess the phenolic composition and antioxidant properties of above- and under-ground parts of P. angulata at four growth stages and evaluate their potential as chemical quality indicators. Methods: A method of HPLC-DAD was used to assess the phenolic profiles. Anti-radical potential, total antioxidant capacity and iron reducing power were evaluated by spectrometric methods. Sample distinctiveness was determined by cluster analyses. RESULTS: The HPLC-DAD analysis revealed 21 phenolic compounds, which were accumulated in a tissue- and age-specific manner. On the contrary, a clear distinctiveness between phenolic contents and antioxidant properties of samples was not observed. Mature flowers highlighted for its total phenolic content (6.50 mg/g fresh tissue) and total antioxidant capacity (416.1 μg/mL). Young leaves were outstanding for its flavonoid content (37.86 μg/g fresh tissue) and reducing power (A700nm = 4.04). Immature calyces were worthy for its phenolic acid concentration (1.28 μg/g fresh tissue) and its free radical scavenging activity (EC50 = 0.12 mg/mL). CONCLUSIONS: Phenolic profiles represent reliable chemical quality indicators for determining plant age, tissular origin, authenticity, and adulteration of herbal preparations of P. angulata.
URI: http://hdl.handle.net/10662/8715
Date: 2017


Files in this item

Files Size Format View
0719-4250_5_2_114.pdf 1.276Mb PDF View  Thumbnail

The following license files are associated with this item:

This item appears in the following Collection(s)

Show full item record

Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International

Search DSpace


Browse

My Account

Statistics

Help

Redes sociales