Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study

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Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study

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Title: Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study
Author: Contreras Aguilar, María Dolores; Escribano Tortosa, Damián; Martín Cuervo, María; Tecles Vicente, Fernando; Cerón Madrigal, Jose Joaquín
Abstract: ANTECEDENTES: El objetivo de este estudio fue evaluar la alfa-amilasa salival (sAA), considerada un biomarcador no invasivo para la actividad del sistema nervioso simpático (SNS), y el cortisol salival como posible biomarcador del estrés inducido por el dolor, en caballos con enfermedad abdominal aguda. Por lo tanto, se realizó un estudio observacional prospectivo en el que se analizaron ambos biomarcadores en un grupo de caballos con síndrome agudo de abdomen y se compararon con un grupo de caballos de control sanos mediante una prueba de t de un estudiante no emparejado. Además, la posible relación entre ambos biomarcadores, la puntuación en las escalas de dolor abdominal agudo equino versión 1 (escala EAAPS-1), la frecuencia cardíaca (HR) y la frecuencia respiratoria (RR), el lactato plasmático, la puntuación del síndrome de respuesta inflamatoria sistémica (SIRS) y la concentración de amiloide sérico A (SAA) se evaluó mediante una prueba de correlación de Spearman. RESULTADOS: Un total de 30 caballos fueron incluidos en el estudio, 19 con enfermedad abdominal aguda diagnosticada como grandes desplazamientos de colon, impactaciones simples de la flexión pélvica, cólicos espasmódicos y enteritis y 11 sanos. actividad de sAA (24.5 veces la mediana, P < 0.0001) y cortisol salival (1).La actividad de los sAA se correlacionó significativamente con la escala EAAPS-1 (r = 0,78; intervalo de confianza[IC] del 95%: 0,38 a 0,89; p < 0,001) y la puntuación del SIRS (r = 0,49; IC del 95%: 0,03 a 0,78; p < 0,05). Ni el sAA ni el cortisol salival se correlacionaron con HR, RR, lactato plasmático y SAA. CONCLUSIONES: Aunque este estudio debe considerarse preliminar, las mediciones de alfa-amilasa en la saliva podrían ser un biomarcador del estrés inducido por el dolor en caballos con enfermedad abdominal aguda.BACKGROUND: The aim of this study was to evaluate salivary alpha-amylase (sAA), considered a non-invasive biomarker for sympathetic nervous system (SNS) activity, and salivary cortisol as possible pain-induced stress biomarker, in horses with acute abdominal disease. Therefore, a prospective observational study was performed in which both biomarkers were analyzed in a group of horses with acute abdomen syndrome, and compared with a group of healthy control horses by an unpaired Student’s t-test. In addition, the possible relationship between both biomarkers, the score in Equine Acute Abdominal Pain scales version 1 (EAAPS-1 scale), Heart Rate (HR) and Respiratory Rate (RR), plasma lactate, the systemic inflammatory response syndrome (SIRS) score and serum amyloid A (SAA) concentration was assessed by a Spearman correlation test. RESULTS: A total of 30 horses were included in the study, 19 with acute abdominal disease diagnosed as large colon displacements, simple impactions of the pelvic flexure, spasmodic colics and enteritis and 11 healthy ones. sAA activity (24.5 median-fold, P < 0.0001) and salivary cortisol (1.7 median-fold, P < 0.01) were significantly higher in horses with acute abdomen than in healthy horses. sAA activity was significantly correlated with EAAPS-1 scale (r = 0.78, 95% confidence interval [CI] 0.38–0.89, P < 0.001) and SIRS score (r = 0.49, 95% CI 0.03–0.78, P < 0.05). Neither sAA nor salivary cortisol correlated with HR, RR, plasma lactate and SAA. CONCLUSIONS: Although this study should be considered as preliminary one, alpha-amylase measurements in saliva could be a biomarker of pain-induced stress in horses with acute abdominal disease.
URI: http://hdl.handle.net/10662/9926
Date: 2018


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