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http://hdl.handle.net/10662/19409
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Campo DC | Valor | idioma |
---|---|---|
dc.contributor.author | Martín Hidalgo, David | - |
dc.contributor.author | Romarowski, Ana | - |
dc.contributor.author | Gervasi, María Gracia | - |
dc.contributor.author | Navarrete, Felipe | - |
dc.contributor.author | Balbach, Melanie | - |
dc.contributor.author | Salicioni, Ana María | - |
dc.contributor.author | Lonny R, Levin | - |
dc.contributor.author | Buck, Jochen | - |
dc.contributor.author | Visconti, Pablo Ernesto | - |
dc.date.accessioned | 2024-01-29T13:25:34Z | - |
dc.date.available | 2024-01-29T13:25:34Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.uri | http://hdl.handle.net/10662/19409 | - |
dc.description.abstract | Mammalian sperm acquire fertilization capacity in the female reproductive tract in a process known as capacitation. During capacitation, sperm change their motility pattern (i.e., hyperactivation) and become competent to undergo the acrosome reaction. We have recently shown that, in the mouse, sperm capacitation is associated with increased uptake of fluorescently labeled deoxyglucose and with extracellular acidification (ECAR) suggesting enhanced glycolysis. Consistently, in the present work we showed that glucose consumption is enhanced in media that support mouse sperm capacitation suggesting up-regulation of glucose metabolic pathways. The increase in glucose consumption was modulated by bicarbonate and blocked by protein kinase A and soluble adenylyl cyclase inhibitors. Moreover, permeable cAMP agonists increase glucose consumption in sperm incubated in conditions that do not support capacitation. Also, the increase in glucose consumption was reduced when sperm were incubated in low calcium conditions. Interestingly, this reduction was not overcome with cAMP agonists. Despite these findings, glucose consumption of sperm from Catsper1 knock-out mice was similar to the one from wild type suggesting that other sources of calcium are also relevant. Altogether, these results suggest that cAMP and calcium pathways are involved in the regulation of glycolytic energy pathways during murine sperm capacitation | es_ES |
dc.description.sponsorship | We are grateful to Dr. Jean-Ju Chung and Dr. David Clapham that originally donated CatSper1 KO mice. This study was supported by NIH grants HD-038082 (to PEV) and HD088571 (to JB, LL and PEV). David M. Hidalgo was recipient of a post-doctoral Grant from the Government of Extremadura (Spain) and by Fondo Social Europeo (PO14005). Ana Romarowski is supported by a fellowship from Lalor Foundation | es_ES |
dc.format.extent | 10 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley Online Library | es_ES |
dc.subject | Espermatozoide | es_ES |
dc.subject | Sperm | es_ES |
dc.subject | Capacitación | es_ES |
dc.subject | Capacitation | es_ES |
dc.subject | ATP | es_ES |
dc.subject | ATP | es_ES |
dc.subject | Glucolisis | es_ES |
dc.subject | Glycolysis | es_ES |
dc.subject | Consumo de glucosa | es_ES |
dc.subject | Glucose consumption | es_ES |
dc.title | Capacitation increases glucose consumption in murine sperm | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | embargoedAccess | es_ES |
dc.subject.unesco | 2401 Biología Animal (Zoología) | es_ES |
dc.date.embargoEndDate | 2025-01-29 | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | N/A | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Biología Vegetal, Ecología y Ciencias de la Tierra | es_ES |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/full/10.1002/mrd.23421 | es_ES |
dc.identifier.doi | 10.1002/mrd.23421 | - |
dc.identifier.publicationtitle | Molecular Reproduction and Development | es_ES |
dc.identifier.publicationfirstpage | 1037 | es_ES |
dc.identifier.publicationlastpage | 1047 | es_ES |
dc.identifier.publicationvolume | 87 | es_ES |
dc.identifier.orcid | 0000-0002-6787-0006 | es_ES |
Colección: | DFSIO - Artículos |
Archivos
Archivo | Descripción | Tamaño | Formato | |
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nihms-1645951.pdf | Artículo versión HHS Public Access | 787,75 kB | Adobe PDF | Descargar |
BOR-1-Copyright-2020.pdf | Carta de aceptación de la revista Biology of Reproduction para publicar el artículo por un año | 134,43 kB | Adobe PDF | Descargar |
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