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dc.contributor.authorMartín Hidalgo, David-
dc.contributor.authorRomarowski, Ana-
dc.contributor.authorGervasi, María Gracia-
dc.contributor.authorNavarrete, Felipe-
dc.contributor.authorBalbach, Melanie-
dc.contributor.authorSalicioni, Ana María-
dc.contributor.authorLonny R, Levin-
dc.contributor.authorBuck, Jochen-
dc.contributor.authorVisconti, Pablo Ernesto-
dc.date.accessioned2024-01-29T13:25:34Z-
dc.date.available2024-01-29T13:25:34Z-
dc.date.issued2020-10-
dc.identifier.urihttp://hdl.handle.net/10662/19409-
dc.description.abstractMammalian sperm acquire fertilization capacity in the female reproductive tract in a process known as capacitation. During capacitation, sperm change their motility pattern (i.e., hyperactivation) and become competent to undergo the acrosome reaction. We have recently shown that, in the mouse, sperm capacitation is associated with increased uptake of fluorescently labeled deoxyglucose and with extracellular acidification (ECAR) suggesting enhanced glycolysis. Consistently, in the present work we showed that glucose consumption is enhanced in media that support mouse sperm capacitation suggesting up-regulation of glucose metabolic pathways. The increase in glucose consumption was modulated by bicarbonate and blocked by protein kinase A and soluble adenylyl cyclase inhibitors. Moreover, permeable cAMP agonists increase glucose consumption in sperm incubated in conditions that do not support capacitation. Also, the increase in glucose consumption was reduced when sperm were incubated in low calcium conditions. Interestingly, this reduction was not overcome with cAMP agonists. Despite these findings, glucose consumption of sperm from Catsper1 knock-out mice was similar to the one from wild type suggesting that other sources of calcium are also relevant. Altogether, these results suggest that cAMP and calcium pathways are involved in the regulation of glycolytic energy pathways during murine sperm capacitationes_ES
dc.description.sponsorshipWe are grateful to Dr. Jean-Ju Chung and Dr. David Clapham that originally donated CatSper1 KO mice. This study was supported by NIH grants HD-038082 (to PEV) and HD088571 (to JB, LL and PEV). David M. Hidalgo was recipient of a post-doctoral Grant from the Government of Extremadura (Spain) and by Fondo Social Europeo (PO14005). Ana Romarowski is supported by a fellowship from Lalor Foundationes_ES
dc.format.extent10 p.es_ES
dc.format.mimetypeapplication/pdfen_US
dc.language.isoenges_ES
dc.publisherWiley Online Libraryes_ES
dc.subjectEspermatozoidees_ES
dc.subjectSpermes_ES
dc.subjectCapacitaciónes_ES
dc.subjectCapacitationes_ES
dc.subjectATPes_ES
dc.subjectATPes_ES
dc.subjectGlucolisises_ES
dc.subjectGlycolysises_ES
dc.subjectConsumo de glucosaes_ES
dc.subjectGlucose consumptiones_ES
dc.titleCapacitation increases glucose consumption in murine spermes_ES
dc.typearticlees_ES
dc.description.versionpeerReviewedes_ES
europeana.typeTEXTen_US
dc.rights.accessRightsembargoedAccesses_ES
dc.subject.unesco2401 Biología Animal (Zoología)es_ES
dc.date.embargoEndDate2025-01-29es_ES
europeana.dataProviderUniversidad de Extremadura. Españaes_ES
dc.type.versionpublishedVersiones_ES
dc.contributor.affiliationN/Aes_ES
dc.contributor.affiliationUniversidad de Extremadura. Departamento de Biología Vegetal, Ecología y Ciencias de la Tierraes_ES
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/mrd.23421es_ES
dc.identifier.doi10.1002/mrd.23421-
dc.identifier.publicationtitleMolecular Reproduction and Developmentes_ES
dc.identifier.publicationfirstpage1037es_ES
dc.identifier.publicationlastpage1047es_ES
dc.identifier.publicationvolume87es_ES
dc.identifier.orcid0000-0002-6787-0006es_ES
Colección:DFSIO - Artículos

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