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http://hdl.handle.net/10662/19914
Títulos: | First detection of Leishmania kDNA in canine cerumen samples by qPCR |
Autores/as: | Belinchón Lorenzo, Silvia Parejo Rosas, Juan Carlos Iniesta, Virginia Fernández Cotrina, Javier Muñoz Madrid, Rubén Monroy, Isabel Baz, Victoria Gómez Luque, Adela Serrano Aguilera, Francisco Javier Barneto, José Luis Gómez Nieto, Luis Carlos |
Palabras clave: | Cerumen;Dog;Kinetoplast DNA (kDNA);Leishmania infantum;Real Time PCR;Perro;ADN del cinetoplasto (ADNk);Leishmania infantil;PCR en tiempo real |
Fecha de publicación: | 2016 |
Editor/a: | ELSEVIER SIENCE BV |
Resumen: | Nowadays, searching for alternative non-invasive methods for molecular diagnosis of canine visceral leishmaniosis is getting increasingly important. We previously described the presence of Leishmania kinetoplast DNA (kDNA) in canine hair; in this case we hypothesized whether foreign DNA might be present in cerumen of dogs with leishmaniosis, and be detected by Real time quantitative PCR (qPCR). A population of 38 dogs that lived in Leishmania endemic areas was divided in two groups: A (33 dogs with confirmed leishmaniosis by serological techniques) and B (5 healthy dogs). Blood, lymph node, bone marrow and cerumen samples from all animals were tested for the presence of parasite kDNA. Our method was 100% specific, and in dogs from group A, Leishmania infantum kDNA was detected and quantified in the 100% of lymph node samples, in 90.9% of cerumen samples, in 88.5% of the bone marrow samples and in 57.6% of the blood samples. The qPCR-cerumen is a new non-invasive method that shows a high potential for the diagnosis of zoonotic visceral leishmaniosis. |
URI: | http://hdl.handle.net/10662/19914 |
ISSN: | 0304-4017 |
DOI: | 10.1016/j.vetpar.2016.05.021 |
Colección: | DENFE - Artículos |
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