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http://hdl.handle.net/10662/20138
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Campo DC | Valor | idioma |
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dc.contributor.author | Gutiérrez Martín, Yolanda | - |
dc.contributor.author | Martín Romero, Francisco Javier | - |
dc.contributor.author | Iñesta Vaquera, Francisco de Asis | - |
dc.contributor.author | Gutiérrez Merino, Carlos | - |
dc.contributor.author | Henao Dávila, Fernando | - |
dc.date.accessioned | 2024-02-07T08:43:48Z | - |
dc.date.available | 2024-02-07T08:43:48Z | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://hdl.handle.net/10662/20138 | - |
dc.description.abstract | The Ca2+-ATPase of skeletal muscle sarcoplasmic reticulum (SERCA), an integral membrane protein, becomes irreversibly inactivated in vitro by the addition of a single bolus of peroxynitrite with a K0.5 of 200–300 µm, and this results in a large decrease of the ATP-dependent Ca2+ gradient across the sarcoplasmic reticulum (SR) membranes. The inactivation of SERCA is raised by treatment of SR vesicles with repetitive micromolar pulses of peroxynitrite. The inhibition of the SERCA is due to the oxidation of thiol groups and tyrosine nitration. Scavengers that react directly with peroxynitrite, such as cysteine, reduced glutathione, NADH, methionine, ascorbate or Trolox, a water-soluble analog of α-tocopherol, afforded significant protection. However, dimethyl sulfoxide and mannitol, two hydroxyl radical scavengers, and α-tocopherol did not protect SERCA from inactivation. Our results showed that the target of peroxynitrite is the cytosolic globular domain of the SERCA and that major skeletal muscle intracellular reductants (ascorbate, NADH and reduced glutathione) protected against inhibition of this ATPase by peroxynitrite. | es_ES |
dc.description.sponsorship | This work has been funded by Grants from the Spanish Ministerio de Ciencia y Tecnología (BMC2000-0547, to C. G.-M.) and from the Consejería de Educación, Ciencia y Tecnología of the Junta de Extremadura (IPR99A019, to F. H. and IPR00A091, to C. G.-M.). Y. G.-M. is the recipient of a Predoctoral Fellowship of the Spanish Ministerio de Educación y Cultura. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.subject | Ca2+-ATPase | es_ES |
dc.subject | Calcium homeostasis | es_ES |
dc.subject | Peroxynitrite | es_ES |
dc.subject | Sarcoplasmic reticulum | es_ES |
dc.subject | Homeostasis del calcio | - |
dc.subject | Peroxinitrito | - |
dc.subject | Retículo sarcoplásmico | - |
dc.title | Modulation of sarcoplasmic reticulum Ca(2+)-ATPase by chronic and acute exposure to peroxynitrite | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | closedAccess | es_ES |
dc.subject.unesco | 2302.21 Biología Molecular | - |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Gutiérrez-Martín Y, Martín-Romero FJ, Iñesta-Vaquera FA, Gutiérrez-Merino C, Henao F. Modulation of sarcoplasmic reticulum Ca(2+)-ATPase by chronic and acute exposure to peroxynitrite. Eur J Biochem. 2004 Jul;271(13):2647-57. doi: 10.1111/j.1432-1033.2004.04193.x. PMID: 15206930. | es_ES |
dc.type.version | acceptedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Bioquímica, Biología Molecular y Genética | es_ES |
dc.relation.publisherversion | https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.2004.04193.x | es_ES |
dc.identifier.doi | 10.1111/j.1432-1033.2004.04193.x | - |
dc.identifier.publicationtitle | European Journal of Biochemistry | es_ES |
dc.identifier.publicationfirstpage | 2647 | es_ES |
dc.identifier.publicationlastpage | 2657 | - |
dc.identifier.publicationvolume | 271(13) | es_ES |
dc.identifier.orcid | 0000-0001-6796-7396 | - |
dc.identifier.orcid | 0000-0003-3673-7007 | - |
dc.identifier.orcid | 0000-0003-1117-8544 | - |
Colección: | DBYBM - Artículos |
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Archivo | Descripción | Tamaño | Formato | |
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