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http://hdl.handle.net/10662/20218
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DC Field | Value | Language |
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dc.contributor.author | Hortigón Vinagre, Maria Pura | - |
dc.contributor.author | Henao Dávila, Fernando | - |
dc.date.accessioned | 2024-02-07T10:40:34Z | - |
dc.date.available | 2024-02-07T10:40:34Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://hdl.handle.net/10662/20218 | - |
dc.description.abstract | Lipid peroxidation (LP), induced by oxidative stress, is associated with degenerative processes. 4-Hydroxy-2-nonenal (HNE), a highly reactive diffusible product of LP, is considered by-product and mediator of oxidative stress. Its level increases under pathological conditions such as cardiovascular diseases. In this study, we partially characterized the mechanisms of HNE-mediated cytotoxicity in cardiomyocytes. After establishing that pathophysiological doses of HNE trigger cell death dependent on the incubation time and dose of HNE (LD50 = 4.4 μM), we tackled the mechanisms that underlie the cell death induced by HNE. Our results indicate that HNE rapidly increases intracellular Ca(2+); it also increases the rate of reactive oxygen species generation and causes a loss of mitochondrial membrane potential (ΔΨm) as well as a decrease in the ATP and GSH levels. Such alterations result in the activation of caspase-3 and DNA breakdown, both characteristic features of apoptotic cell death, as well as disruption of the cytoskeleton. Moreover, the nucleophilic compounds N-acetyl-cysteine and β-mercapto-propionyl-glycine, and the synthetic antioxidant Trolox exert a potent antioxidant action against HNE damage; this suggests its use as effective compounds in order to reduce the damage occurred as consequence of cardiovascular disorders in which oxidative stress and hence LP take place. | es_ES |
dc.description.sponsorship | Technical and human support provided by Facility of Bioscience Applied Techniques (STAB) of Servicios de Apoyo a la Investigación (SAIUEx) (financed by Universidad de Extremadura, Junta de Extremadura, Ministerio de Ciencia e Innovación, Fondo Europeo de Desarrollo Regional-FEDER). This work was supported by a Grant from the Junta de Extremadura, Spain (PRI06A132 to F. Henao; GRU09056 and GRU10046 to Grupo de Investigación en Enfermedades Neurodegenerativas). M.P.H.-V. had been the recipient of predoctoral fellowships from the Junta de Extremadura (Spain). All Spanish funding is co-sponsored by the European Union FEDER (Fondo Europeo de Desarrollo Regional) program. | - |
dc.format.extent | 13 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cardiomyocytes | es_ES |
dc.subject | 4-Hydroxy-2-nonenal | es_ES |
dc.subject | Calcium | es_ES |
dc.subject | ROS | es_ES |
dc.subject | Mitochondria | es_ES |
dc.subject | Apoptosis | es_ES |
dc.subject | Cardiomiocitos | - |
dc.subject | 4-hidroxi-2-nonenal | - |
dc.subject | Calcio | - |
dc.subject | Mitocondrias | - |
dc.title | Apoptotic cell death in cultured cardiomyocytes following exposure to low concentrations of 4-hydroxy-2-nonenal | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | closedAccess | es_ES |
dc.subject.unesco | 3205.01Cardiología | - |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Hortigón-Vinagre, M.P., Henao, F. Muerte celular apoptótica en cardiomiocitos cultivados tras exposición a bajas concentraciones de 4-hidroxi-2-nonenal. Cardiovasc Toxicol 14, 275–287 (2014). https://doi.org/10.1007/s12012-014-9251-5 | - |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Bioquímica, Biología Molecular y Genética | es_ES |
dc.relation.publisherversion | https://link.springer.com/article/10.1007/s12012-014-9251-5 | es_ES |
dc.identifier.doi | 10.1007/s12012-014-9251-5 | - |
dc.identifier.publicationtitle | Cardiovascular toxicology | es_ES |
dc.identifier.publicationfirstpage | 275 | es_ES |
dc.identifier.publicationlastpage | 287 | es_ES |
dc.identifier.publicationvolume | 14 (3) | es_ES |
dc.identifier.orcid | 0000-0001-5531-1779 | es_ES |
Appears in Collections: | DBYBM - Artículos |
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