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http://hdl.handle.net/10662/20273
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Campo DC | Valor | idioma |
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dc.contributor.author | Clementi, Marisa | - |
dc.contributor.author | Sánchez, Catherine | - |
dc.contributor.author | Benitez López, Dixan Agustín | - |
dc.contributor.author | Contreras, Héctor | - |
dc.contributor.author | Huidobro, Christian | - |
dc.contributor.author | Cabeza, Juan | - |
dc.contributor.author | Acevedo, Cristian | - |
dc.contributor.author | Castellón, Enrique | - |
dc.date.accessioned | 2024-02-07T11:35:05Z | - |
dc.date.available | 2024-02-07T11:35:05Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | http://hdl.handle.net/10662/20273 | - |
dc.description.abstract | Background: Gonadotropin-releasing-hormone (GnRH) analogs are widely used to block hypothalamic-pituitary-gonadal axis and inhibit blood androgen levels in patients with prostate cancer (PCa). In addition, GnRH analogs induce proliferation arrest and apoptosis through GnRH receptors expressed on the membrane of PCa cells. Possible molecular mechanisms involved in GnRH-mediated apoptosis on prostate cancer cells were studied. Methods: Primary cultures from PCa and benign prostatic hyperplasia (BPH) (non-malignant control) were derived from samples provided by our Institutional Hospital. Cell cultures were incubated for 24 hr with 20 ng/ml of GnRH agonist Leuprolide (Lp) or antagonist Cetrorelix (Cx). Apoptosis was evaluated by studying the expression of Bax and Bcl-2 and the activation of caspase-9 (intrinsic pathway), caspase-8 (extrinsic pathway), and caspase-3. Also, mRNA level, protein expression and phosphorylation of p53 were studied. Results: Cleaved caspase-8 and -3, but not -9, increased in presence of Lp and Cx in PCa cell cultures. Bax and Bcl-2 mRNA levels showed no changes after GnRH-analog treatments. Only Bax protein showed an increase after Cx treatment in PCa cell cultures. p53 mRNA level was higher in PCa than in BPH cell cultures. Lp and Cx increased p53 expression and phosphorylation in PCa cell cultures. Conclusions: Apoptosis induced by GnRH analogs seems to be mediated by extrinsic pathway involving p53 phosphorylation. Phosphorylated-p53 might be associated with the increase in apoptotic NGF receptor, p75, previously reported by our laboratory. These findings reinforce the concept of clinical use of GnRH analogs for PCa suggesting that intraprostatic treatment may be more effective. | es_ES |
dc.description.sponsorship | This work was supported by Fondo Nacional de Ciencia y Tecnología (FONDECYT) project 1020969 and Fellowship PG 102003 U. de Chile (MC). | - |
dc.format.extent | 9 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley-Liss | es_ES |
dc.subject | Prostate cancer | es_ES |
dc.subject | p53 | es_ES |
dc.subject | Apoptosis | es_ES |
dc.subject | Primary cell culture | es_ES |
dc.subject | GnRH analogs | es_ES |
dc.subject | Cáncer de próstata | - |
dc.subject | Análogos de GnRH | - |
dc.subject | Cultivo celular primario | - |
dc.title | Gonadotropin releasing hormone analogs induce apoptosis by extrinsic pathway involving p53 phosphorylation in primary cell cultures of human prostatic adenocarcinomas | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | closedAccess | es_ES |
dc.subject.unesco | 3201.01Oncología | - |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Clementi M, Sánchez C, Benitez DA, Contreras HR, Huidobro C, Cabezas J, Acevedo C, Castellón EA. Gonadotropin releasing hormone analogs induce apoptosis by extrinsic pathway involving p53 phosphorylation in primary cell cultures of human prostatic adenocarcinomas. Prostate. 2009 Jul 1;69(10):1025-33. doi: 10.1002/pros.20954. PMID: 19301301. | es_ES |
dc.identifier.bibliographicCitation | Clementi, M., Sánchez, C., Benitez, D.A., Contreras, H.R., Huidobro, C., Cabezas, J., Acevedo, C. and Castellón, E.A. (2009), Gonadotropin releasing hormone analogs induce apoptosis by extrinsic pathway involving p53 phosphorylation in primary cell cultures of human prostatic adenocarcinomas. Prostate, 69: 1025-1033. https://doi.org/10.1002/pros.20954 | - |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Bioquímica, Biología Molecular y Genética | es_ES |
dc.contributor.affiliation | Universidad de Chile | - |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1002/pros.20954 | es_ES |
dc.identifier.doi | 10.1002/pros.20954 | - |
dc.identifier.publicationtitle | Prostate | es_ES |
dc.identifier.publicationissue | 69 | es_ES |
dc.identifier.publicationfirstpage | 1025 | es_ES |
dc.identifier.publicationlastpage | 1033 | es_ES |
dc.identifier.orcid | 0009-0005-7398-042X | es_ES |
Colección: | DBYBM - Artículos |
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