Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10662/20305
Títulos: The sirtuin 1 activator YK 3-237 stimulates capacitation-related events in human spermatozoa
Autores/as: Martín Hidalgo, David
González Fernández, Lauro
Bragado González, María Julia
García Marín, Luis Jesús
Alves, Marco G.
Oliveira, Pedro F.
Palabras clave: Espermatozoide;Sperm;Sirtuina;Sirtuin;Capacitación;Capacitation;Reacción crosómica;Acrosome reaction;Fosforilación tirosina;Protein tyrosine phosphorylation
Fecha de publicación: 2023
Editor/a: Elsevier
Resumen: Research question: Does sirtuin-1 (SIRT1) have a role in the human spermatozoa capacitation process? Design: Human spermatozoa were incubated for 6 h in a capacitating medium in presence or absence of the specific SIRT1 activator, YK 3-237. Several sperm parameters were determined by flow cytometry: viability, acrosome reaction and mitochondria membrane status. Sperm motility was determined objectively by computer-assisted semen analysis. Sperm capacitation status was evaluated by the extent of protein tyrosine phosphorylation and by the percentage of spermatozoa with the acrosome reacted by a calcium ionophore challenge. Results: SIRT1 was detected in the connecting piece of human spermatozoa where a lysine acetylation pattern was mainly found along the sperm tail. SIRT1 activation accelerates the occurrence of a phenotype associated with human sperm capacitation, with no differences seen in the lysine acetylation pattern. After 1 h of co-incubation of YK 3-237 with human spermatozoa, tyrosine phosphorylation levels were comparable to control levels after 6 h of incubation in capacitating conditions. In addition, the activator improved sperm responsiveness to a Ca2+ ionophore (A23187) challenge determined by an increase in acrosome-reacted spermatozoa (P = 0.025). Importantly, sperm viability and mitochondrial activity-related parameters assessed by flow cytometry were not affected by YK 3-237. Conclusion: YK 3-237 induces capacitation-related events in human spermatozoa such an increase of tyrosine phosphorylation levels and acrosome-reacted spermatozoa after the ionophore challenge. Together, these results show that YK 3-237 affects human spermatozoa capacitation-related events by a mechanism independent of protein lysine acetylation but dependent on bicarbonate and calcium
Descripción: Versión aceptada del trabajo publicado en: Reproductive BioMedicine Online, 46, 1, 165-178. ISSN 1472-6483. https://doi.org/10.1016/j.rbmo.2022.07.011
URI: http://hdl.handle.net/10662/20305
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2022.07.011
Colección:DFSIO - Artículos
INBIO G+C - Artículos

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