Identificador persistente para citar o vincular este elemento:
http://hdl.handle.net/10662/20513
Títulos: | Caspase 3 activation in human spermatozoa in response to hydrogen peroxide and progesterona |
Autores/as: | Bejarano Hernando, Ignacio Lozano Cordero, Graciela Ortiz Ruiz, Águeda García Malpartida, Juan Francisco Paredes Royano, Sergio Damián Rodríguez Moratinos, Ana Beatriz Pariente Llanos, José Antonio |
Palabras clave: | Apoptosis;Spermatozoa;Caspases;Caspasas;Espermatozoides |
Fecha de publicación: | 2008 |
Editor/a: | Elsevier |
Resumen: | Objective To determine the role of calcium signaling on apoptosis evoked by the reactive oxygen species H2O2 and by the physiological agonist P in human ejaculated spermatozoa. Design Laboratory study. Setting Center for assisted human reproduction in a hospital in Spain. Patient(s) Forty-five healthy volunteers. Intervention(s) Spermatozoa were treated with increasing concentrations of hydrogen peroxide (H2O2; 10 μM, 100 μM, and 1 mM) or with 20 μM of P for 5–120 minutes. Main Outcome Measure(s) Activation of caspase-3 and -9 as well as phosphatidylserine externalization were examined in human ejaculated spermatozoa by fluorescence methods. Result(s) Hydrogen peroxide and P induced activation of caspase-3 and -9. In addition, the effect of H2O2 and P was time dependent. Dimethyl-1,2-bis (aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid loading was able to inhibit H2O2- and P-induced caspase-3 activation and phosphatidylserine externalization. Pretreatment of spermatozoa with Ru360, to block the calcium uptake into mitochondria, also was able to decrease the activation of caspase-3 and phosphatidylserine exposure that was stimulated by either H2O2 or P. Conclusion(s) These findings suggest that H2O2- and P-induced mitochondrial apoptosis is dependent on calcium signaling. |
URI: | http://hdl.handle.net/10662/20513 |
ISSN: | 0015-0282 |
DOI: | 10.1016/j.fertnstert.2007.08.069 |
Colección: | DFSIO - Artículos |
Archivos
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
j_fertnstert_2007_08_069.pdf | 267,67 kB | Adobe PDF | Descargar |
Este elemento está sujeto a una licencia Licencia Creative Commons