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http://hdl.handle.net/10662/21432
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Campo DC | Valor | idioma |
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dc.contributor.author | Becerro Rey, Laura | - |
dc.contributor.author | Martín Cano, Francisco Eduardo | - |
dc.contributor.author | Ortega Ferrusola, Cristina | - |
dc.contributor.author | Rodríguez Martínez, Heriberto | - |
dc.contributor.author | Gaitskell Phillips, Gemma | - |
dc.contributor.author | Álvarez, Eva Da Silva | - |
dc.contributor.author | Silva Rodríguez, Antonio | - |
dc.contributor.author | Gil Anaya, María Cruz | - |
dc.contributor.author | Peña Vega, Fernando Juan | - |
dc.date.accessioned | 2024-06-06T10:02:28Z | - |
dc.date.available | 2024-06-06T10:02:28Z | - |
dc.date.issued | 2023 | - |
dc.identifier.issn | 2047-2919 | - |
dc.identifier.uri | http://hdl.handle.net/10662/21432 | - |
dc.description.abstract | Background: Most commerce of equine seminal doses is carried out using commercial extenders under refrigeration at 5◦C. Objectives: To determine if 10 mM pyruvate in a 67 mM glucose extender and storage at 22◦C could be the basis of an alternative storage method to cooling to 5◦C. Material and methods: Stallion ejaculates were extendedin: INRA96 (67 mM glucose, non-pyruvate control), modified Tyrode’s (67 mM glu-cose–10 mM pyruvate), supplemented with 0, 10, 50, and 100 μM itaconate. As itaconate was vehiculated in DMSO, a control vehicle was also included. Sperm motility, viability, mitochondrial membrane potential, and production of reactive oxygen species were measured after collection and again after 48 and 96 h of storage at 22◦C. To disclose molecular metabolic changes, spermatozoa were incubated up to 3 h in modi-fied Tyrode’s 67mM glucose–10mM pyruvate and modified Tyrode’s 67mM glucose, andmetabolic analysis conducted. Results: After 96 h of storage aliquots stored in the control, INRA96 had a very poor total motil-ity of 5.6% } 2.3%, while in the 67 mM glucose–10 mM pyruvate/10 μM itaconate extender, total motility was 34.7% } 3.8% (p = 0.0066). After 96 h, viability was better in most pyruvate-based media, and the mitochondrial membrane potential in spermatozoa extended in INRA96 was relatively lower (p < 0.0001). Metabolomics revealed that in the spermatozoa incubated in the high pyruvate media, there was an increase in the relative amounts of NAD+, pyruvate, lactate, and ATP. Discussion and conclusions: Aliquots stored in a 67 mM glucose–10 mM pyruvatebased medium supplemented with 10 μM itaconate, maintained a 35% total motility after 96 h of storage at 22◦C, which is considered the minimum acceptable motility for com-mercialization. Improvements may be related to the conversion of pyruvate to lactate and regeneration of NAD+. | es_ES |
dc.description.sponsorship | The authors received financial support for this study from the Ministerio de Ciencia-European Fund for Regional Development (EFRD), Madrid, Spain (grant PID2021-122351OB-I00), Junta de Extremadura-FEDER (IB 20008), and Re-search Council FORMAS (Project 2019-00288), Stockholm, Sweden. Laura Becerro-Rey holds a PhD grant from the Ministry of Science, Madrid, Spain (PRE2022- 103090). | es_ES |
dc.format.extent | 16 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Inseminación artificial | es_ES |
dc.subject | Equino | es_ES |
dc.subject | Extensor | es_ES |
dc.subject | Piruvato | es_ES |
dc.subject | Espermatozoides | es_ES |
dc.subject | Artificial insemination | es_ES |
dc.subject | Equine | es_ES |
dc.subject | Extender | es_ES |
dc.subject | Pyruvate | es_ES |
dc.subject | Spermatozoa | es_ES |
dc.title | Aging of stallion spermatozoa stored in vitro is de-layed at 22ºC using a 67mM glucose–10mM py-ruvate-based media | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | openAccess | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3109.07 Patología | es_ES |
dc.subject.unesco | 3104.11 Reproducción | es_ES |
dc.subject.unesco | 3104.05 Équidos | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Becerro Rey, L., Martín Cano, F.E., Ortega Ferrusola, C., Rodríguez-Martínez, H., Gaitskell Phillips, C., Álvarez, E. da S., Silva-Rodríguez, A., Cruz Gil, M., Peña Vega, F.J.(2023). Aging of stallion spermatozoa stored in vitro is delayed at 22ºC using a 67mM glu-cose–10mM pyruvate-based media. Andrology, 2023, 1-16. https://onlinelibrary.wiley.com/doi/full/10.1111/andr.13565 | es_ES |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Medicina Animal | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Química Analítica | es_ES |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/full/10.1111/andr.13565 | es_ES |
dc.identifier.doi | 10.1111/andr.13565 | - |
dc.identifier.publicationtitle | Andrology | es_ES |
dc.identifier.publicationfirstpage | 13565-1 | es_ES |
dc.identifier.publicationlastpage | 13565-16 | es_ES |
dc.identifier.e-issn | 2047-2927 | - |
dc.identifier.orcid | 0009-0007-6984-0760 | es_ES |
dc.identifier.orcid | 0000-0003-4971-4883 | es_ES |
dc.identifier.orcid | 0000-0002-7698-8735 | es_ES |
dc.identifier.orcid | 0000-0002-5194-2124 | es_ES |
dc.identifier.orcid | 0000-0003-4308-7903 | es_ES |
dc.identifier.orcid | 0009-0009-8939-9647 | es_ES |
dc.identifier.orcid | 0000-0001-7041-9673 | es_ES |
Colección: | DMANI - Artículos |
Archivos
Archivo | Descripción | Tamaño | Formato | |
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andr_13565.pdf | 3,55 MB | Adobe PDF | Descargar |
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