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http://hdl.handle.net/10662/7143
Títulos: | PiRNA-associated proteins and retrotransposons are differentially expressed in murine testis and ovary of aryl hydrocarbon receptor deficient mice |
Autores/as: | Rico Leo, Eva María Moreno Marín, Nuria González Rico, Francisco Javier Barrasa Ardila, Eva María Ortega Ferrusola, Cristina Martín Muñoz, Patricia Sánchez Guardado, Luis Óscar Llano Cuadro, Elena Álvarez Barrientos, Alberto Infante Campos, Ascensión Catalina Fernández, Inmaculada Hidalgo Sánchez, Matías Rooij, Dirk G. de Pendás, Alberto M. Peña Vega, Fernando Juan Merino Fernández, Jaime María Fernández Salguero, Pedro María |
Palabras clave: | Receptor de hidrocarburos aril;Proteínas de Nuage;Espermatogénesis;Ovario;Fertilidad;Elementos repetitivos;Aryl hydrocarbon receptor;Nuage proteins;Spermatogenesis;Ovary;Fertility;Repetitive elements |
Fecha de publicación: | 2016 |
Editor/a: | The Royal Society |
Resumen: | Previous studies suggested that the aryl hydrocarbon receptor (AhR) contributes to mice reproduction and fertility. However, the mechanisms involved remain mostly unknown. Retrotransposon silencing by Piwi-interacting RNAs (piRNAs) is essential for germ cell maturation and, remarkably, AhR has been identified as a regulator of murine B1-SINE retrotransposons. Here, using littermate AhR+/+ and AhR-/- mice, we report that AhR regulates the general course of spermatogenesis and oogenesis by a mechanism likely to be associated with piRNA-associated proteins, piRNAs and retrotransposons. piRNA-associated proteins MVH and Miwi are upregulated in leptotene to pachytene spermatocytes with a more precocious timing in AhR-/- than in AhR+/+ testes. piRNAs and transcripts from B1-SINE, LINE-1 and IAP retrotransposons increased at these meiotic stages in AhR-null testes. Moreover, B1-SINE transcripts colocalize with MVH and Miwi in leptonema and pachynema spermatocytes. Unexpectedly, AhR-/- males have increased sperm counts, higher sperm functionality and enhanced fertility than AhR+/+ mice. In contrast, piRNA-associated proteins and B1-SINE and IAP-derived transcripts are reduced in adult AhR-/- ovaries. Accordingly, AhR-null female mice have lower numbers of follicles when compared with AhR+/+ mice. Thus, AhR deficiency differentially affects testis and ovary development possibly by a process involving piRNA-associated proteins, piRNAs and transposable elements. |
URI: | http://hdl.handle.net/10662/7143 |
ISSN: | 2046-2441 |
DOI: | 10.1098/rsob.160186 |
Colección: | DBYBM - Artículos |
Archivos
Archivo | Descripción | Tamaño | Formato | |
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rsob_160186.pdf | 2,51 MB | Adobe PDF | Descargar |
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