Please use this identifier to cite or link to this item: http://hdl.handle.net/10662/24069
Title: Evaluation of the extract of “Pseudopithomyces chartarum” to be used as biocontrol agent against “Phytophthora cinnamomi” in “Lupinus luteus”
Authors: García-Latorre Nieto, Carlos
Morales Rodrigo, Sara
Santamaría Becerril, Óscar
Keywords: Hongos endófitos;Metabolitos secundarios;Protección vegetal;Pseudopithomyces chartarum;Lupinus luteus;Phytophthora cinnamomi;Fungal endophytes;Secondary metabolites;Plant protection
Issue Date: 2024
Publisher: Springer Nature
Abstract: Endophytic fungi offer promising opportunities for a sustainable disease management in agricultural systems, often related to their secondary metabolites. In this study, the potential of the metabolites produced in vitro by “Pseudopithomyces chartarum” to protect the forage crop “Lupinus luteus” against the soil-borne pathogen “Phytophthora cinnamomi” was evaluated in different in vitro and in planta tests. The fungal extracts containing the metabolites were applied by two different procedures: to seeds by immersion (mycopriming) and to seedlings by spraying (post-emergence). The antagonism between the endophyte fitrates/extracts and the pathogen was studied by dual culture assays and the minimal inhibitory concentration. Other filtrate/extract traits, such as its phytohormones production and nutrient mobilization, were also analyzed. The metabolites contained in the extract were tentatively identified by mass spectrometry. The endophyte’s filtrates and extracts exhibited significant inhibition on the “P. cinnamomi” growth in vitro. Additionally, seed mycopriming and post-emergence extract application positively influenced seed germination, reduced disease severity, and enhanced several growth parameters in treated seedlings. Auxins and gibberellins production, and phosphate solubilization activity were observed in the endophyte, traits potentially contributing to its biocontrol efficacy. The bioactive compounds ciclo(L-Phe-L-Pro), dihydroabikoviromycin, medelamine A, herniarin, and Piptamine, all with antimicrobial properties, were tentatively identified in the extracts, but further research is needed to confirm this identity and to evaluate the extract efficacy under field conditions. This study highlights the potential of certain endophytic fungi as sustainable and environmentally friendly alternatives for a disease management in agriculture.
Description: Financiación Open Access proporcionada gracias al acuerdo CRUE-CSIC con Springer Nature
URI: http://hdl.handle.net/10662/24069
ISSN: 0718-9508
DOI: 10.1007/s42729-024-01969-6
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