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dc.contributor.authorGonzález Fernández, Lauro-
dc.contributor.authorSánchez-Calabuig, M.J.-
dc.contributor.authorAlves, M.G.-
dc.contributor.authorOliveira, P.F.-
dc.contributor.authorMacedo, S.-
dc.contributor.authorGutiérrez-Adán, A.-
dc.contributor.authorRocha, Afonso Duarte dos Reis-
dc.contributor.authorMacías García, Beatriz-
dc.descriptionPublicado en: Reproduction, Fertility and Development 30(2) 297-306 Submitted: 4 November 2016 Accepted: 6 June 2017 Published: 6 July 2017es_ES
dc.description.abstractEquine cumulus-oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This divergence could be related to different glucose metabolism. To test this hypothesis eCOCs, cCOCs, and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30 hours and the maturation rate, glucose metabolism, and expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. Significant differences were found between eCOCs and cCOCs maturation rates (50% vs. 21.7 %; n = 192 and 46 respectively, p < 0.001), glucose consumption (1.8 ± 0.5 vs. 27.9 ± 5.9 nmol/COC; mean ± SEM), pyruvate production (0.1 ± 0.0 vs. 2.4 ± 0.8 nmol/COC; mean ± SEM) and lactate production (4.7 ± 1.3 vs. 64.1 ± 20.6 nmol/COC; mean ± SEM) respectively (p < 0.05). Moreover, similar glucose consumption was observed for EC and CC. Hyaluronan binding protein (TNFAIP6) expression was increased in eCOCs and EC, solute carrier family 2 (facilitated glucose transporter) member 1 (SLC2A1) was increased in eCOCs, while glycolysis-related enzymes and solute carrier family 2 (facilitated glucose transporter) member 3 (SLC2A3) expression did not vary between COCs or mural granulosa cell type. Our data demonstrate that metabolic and genomic differences exist between eCOCs and cCOCs and mural granulosa cells in the horse.es_ES
dc.description.sponsorshipThis work was financed by AGL2015-66145-R funding from the Spanish Ministry of Economy, Industry and 364 Competitiveness and by AGL2015-73249-JIN(AEI/FEDER/UE) from the "Agencia Estatal de Investigación" 365 (AEI) (Spanish Ministry of Economy, Industry and Competitiveness) and "Fondo Europeo de Desarrollo 366 Regional" (FEDER). Beatriz Macías-García holds a postdoctoral grant "Juan de la Cierva Incorporación"(IJCI-367 2014-19428) from the Spanish Ministry of Economy, Industry and Competitiveness. L.G.-F. (Grant reference: 368 SFRH/BPD/85532/2012) and B. M.-G. (Grant reference: SFRH/BPD/84354/2012) were also partially funded by 369 Fundação para a Ciência e a Tecnologia (Portuguese Ministry for Science, Technology and Higher Education) co370 funded by Programa Operacional Potencial Humano (POPH) financed by European Social Fund (ESF) and 371 Portuguese national funds from Ministry for Science, Technology and Higher Education. The authors thank 372 CECA/ICETA (University of Porto) for funding the abattoir dislocations. The collaboration of Linda Rosa 373 Abattoir is highly appreciated. The authors wish to thank the Laboratory of Applied Physiology (Department of 374 Aquatic Production) of the ICBAS (University of Porto) and especially Mariana Hinzmann for allowing us to use 375 their fluorescence microscope. RNAlater was kindly provided by Dr. Michael Jowers.es_ES
dc.format.extent25 p.es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectMaduración in vitroes_ES
dc.subjectResonancia magnética nucleares_ES
dc.subjectIn vitro maturationes_ES
dc.subjectNuclear magnetic resonancees_ES
dc.titleExpanded equine cumulus-oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus-oocyte complexeses_ES
dc.subject.unesco3104.11 Reproducciónes_ES
europeana.dataProviderUniversidad de Extremadura. Españaes_ES
dc.contributor.affiliationUniversidad de Extremadura. Grupo de investigación "Señalización Intracelular y Tecnología de la Reproducción" (SINTREP)es_ES
dc.contributor.affiliationUniversidad de Extremadura. Departamento de Fisiología-
dc.contributor.affiliationUniversidade do Porto. Portugal-
dc.contributor.affiliationInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC)-
dc.contributor.affiliationCentro de Cirugía de Mínima Invasión Jesús Usón. Cáceres-
dc.identifier.publicationtitleReproduction, Fertility and Developmentes_ES
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