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Title: A novel frizzled-based screening tool identifies genetic modifiers of planar cell polarity in drosophila wings
Authors: Carvajal González, José María
Mulero Navarro, Sonia María
Smith, Michael
Mlodzik, Marek
Keywords: Polaridad celular planar;Drosophila;Frizzled;Krasavietz;Parada corta;Planar cell polarity;Short-stop
Issue Date: 2016
Publisher: Genetics Society of America
Abstract: Most mutant alleles in the Fz-PCP pathway genes were discovered in classic Drosophila screens looking for recessive loss-of-function (LOF) mutations. Nonetheless, although Fz-PCP signaling is sensitive to increased doses of PCP gene products, not many screens have been performed in the wing under genetically engineered Fz overexpression conditions, mostly because the Fz phenotypes were strong and/or not easy to score and quantify. Here, we present a screen based on an unexpected mild Frizzled gain-of-function (GOF) phenotype. The leakiness of a chimeric Frizzled protein designed to be accumulated in the endoplasmic reticulum (ER) generated a reproducible Frizzled GOF phenotype in Drosophila wings. Using this genotype, we first screened a genome-wide collection of large deficiencies and found 16 strongly interacting genomic regions. Next, we narrowed down seven of those regions to finally test 116 candidate genes. We were, thus, able to identify eight new loci with a potential function in the PCP context. We further analyzed and confirmed krasavietz and its interactor short-stop as new genes acting during planar cell polarity establishment with a function related to actin and microtubule dynamics.
ISSN: 2160-1836
DOI: 10.1534/g3.116.035535
Appears in Collections:DBYBM - Artículos

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