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Title: PiRNA-associated proteins and retrotransposons are differentially expressed in murine testis and ovary of aryl hydrocarbon receptor deficient mice
Authors: Rico Leo, Eva María
Moreno Marín, Nuria
González Rico, Francisco Javier
Barrasa Ardila, Eva María
Ortega Ferrusola, Cristina
Martín Muñoz, Patricia
Sánchez Guardado, Luis Óscar
Llano Cuadro, Elena
Álvarez Barrientos, Alberto
Infante Campos, Ascensión
Catalina Fernández, Inmaculada
Hidalgo Sánchez, Matías
Rooij, Dirk G. de
Pendás, Alberto M.
Peña Vega, Fernando Juan
Merino Fernández, Jaime María
Fernández Salguero, Pedro María
Keywords: Receptor de hidrocarburos aril;Proteínas de Nuage;Espermatogénesis;Ovario;Fertilidad;Elementos repetitivos;Aryl hydrocarbon receptor;Nuage proteins;Spermatogenesis;Ovary;Fertility;Repetitive elements
Issue Date: 2016
Publisher: The Royal Society
Abstract: Previous studies suggested that the aryl hydrocarbon receptor (AhR) contributes to mice reproduction and fertility. However, the mechanisms involved remain mostly unknown. Retrotransposon silencing by Piwi-interacting RNAs (piRNAs) is essential for germ cell maturation and, remarkably, AhR has been identified as a regulator of murine B1-SINE retrotransposons. Here, using littermate AhR+/+ and AhR-/- mice, we report that AhR regulates the general course of spermatogenesis and oogenesis by a mechanism likely to be associated with piRNA-associated proteins, piRNAs and retrotransposons. piRNA-associated proteins MVH and Miwi are upregulated in leptotene to pachytene spermatocytes with a more precocious timing in AhR-/- than in AhR+/+ testes. piRNAs and transcripts from B1-SINE, LINE-1 and IAP retrotransposons increased at these meiotic stages in AhR-null testes. Moreover, B1-SINE transcripts colocalize with MVH and Miwi in leptonema and pachynema spermatocytes. Unexpectedly, AhR-/- males have increased sperm counts, higher sperm functionality and enhanced fertility than AhR+/+ mice. In contrast, piRNA-associated proteins and B1-SINE and IAP-derived transcripts are reduced in adult AhR-/- ovaries. Accordingly, AhR-null female mice have lower numbers of follicles when compared with AhR+/+ mice. Thus, AhR deficiency differentially affects testis and ovary development possibly by a process involving piRNA-associated proteins, piRNAs and transposable elements.
ISSN: 2046-2441
DOI: 10.1098/rsob.160186
Appears in Collections:DBYBM - Artículos

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