Redox chemistry of the molecular interactions between tea catechins and human serum proteins under simulated hyperglycemic conditions

DSpace/Manakin Repository

español português english

Redox chemistry of the molecular interactions between tea catechins and human serum proteins under simulated hyperglycemic conditions

Show full item record

Title: Redox chemistry of the molecular interactions between tea catechins and human serum proteins under simulated hyperglycemic conditions
Author: Özyurt, Hazal; Luna Estellés, Carolina Luisa; Estévez García, Mario
Abstract: La carbonilación es una modificación irreversible de proteínas oxidadas que ha sido directamente relacionada con una serie de alteraciones de la salud incluida la diabetes tipo 2. Los antioxidantes dietéticos han sido propuestos para contrarrestar el estrés oxidativo que ocurre bajo condiciones de hiperglucemia. Una comprensión de la naturaleza y de las consecuencias de las interacciones moleculares entre los fitoquímicos y proteínas plasmáticas humanas es de sumo interés científico. Tres catequinas del té: epicatechin (CE), galato (CGE) y-3-galato de epigalocatequina (EGCG) fueron probados por (1) su afinidad para unirse a la albúmina sérica humana (ASH) y hemoglobina humana (HH) y (2) su capacidad para inhibir el triptófano (TRP), agotamiento y por la formación de Carbonilos proteicos y pentosidine específicos en las mencionadas proteínas. Ambas proteínas (20 mg mL-1) pudieron reaccionar con concentraciones plasmáticas postprandial de las catequinas (CE: 0,7 μM, CGE: 1,8 μM, y EGCG: 0,7 μM) bajo condiciones simuladas de hiperglucemia (12 mM/0,2 mM de glucosa Fe3 /37 °C/10 días). Las tres catequinas fueron capaces de inhibir la oxidación del PRT y la proteína carbonilación en ambas proteínas plasmáticas. Algunas propiedades de anti-glicación estaban unidas a sus afinidades vinculantes. Las interacciones moleculares reportadas en el presente estudio pueden explicar los supuestos efectos beneficiosos de las catequinas del té contra el deterioro redox vinculados a condiciones de hiperglucemia.Carbonylation is an irreversible modification in oxidized proteins that has been directly related to a number of health disorders including Type 2 diabetes. Dietary antioxidants have been proposed to counteract the oxidative stress occurring under hyperglycemic conditions. An understanding of the nature and consequences of the molecular interactions between phytochemicals and human plasma proteins is of utmost scientific interest. Three tea catechins namely epicatechin (EC), epigallocatechin (EGC) and epigallocatechin-3-gallate (EGCG) were tested for (i) their affinity to bind to human serum albumin (HSA) and human hemoglobin (HH) and (ii) their ability to inhibit tryptophan (Trp) depletion and for the formation of specific protein carbonyls and pentosidine in the aforementioned proteins. Both proteins (20 mg mL−1) were allowed to react with postprandial plasmatic concentrations of the catechins (EC: 0.7 μM, EGC: 1.8 μM, and EGCG: 0.7 μM) under simulated hyperglycemic conditions (12 mM glucose/0.2 mM Fe3+/37 °C/10 days). The three catechins were able to inhibit Trp oxidation and protein carbonylation in both plasma proteins. Some anti-glycation properties were linked to their binding affinities. The molecular interactions reported in the present study may explain the alleged beneficial effects of tea catechins against the redox impairment linked to hyperglycemic conditions.
URI: http://hdl.handle.net/10662/7561
Date: 2016


Files in this item

Files Size Format View
c5fo01525a.pdf 548.7Kb PDF View  Thumbnail

The following license files are associated with this item:

This item appears in the following Collection(s)

Show full item record

Atribución-NoComercial 3.0 España Except where otherwise noted, this item's license is described as Atribución-NoComercial 3.0 España

Search DSpace


Browse

My Account

Statistics

Help

Redes sociales