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http://hdl.handle.net/10662/21386
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DC Field | Value | Language |
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dc.contributor.author | Martín Cano, Francisco Eduardo | - |
dc.contributor.author | Gaitskell Phillips, Gemma | - |
dc.contributor.author | Becerro Rey, Laura | - |
dc.contributor.author | Álvarez, Eva Da Silva | - |
dc.contributor.author | Masot Gómez-Landero, Antonio Javier | - |
dc.contributor.author | Redondo García, Eloy | - |
dc.contributor.author | Silva Rodríguez, Antonio | - |
dc.contributor.author | Ortega Ferrusola, Cristina | - |
dc.contributor.author | Gil Anaya, María Cruz | - |
dc.contributor.author | Peña Vega, Fernando Juan | - |
dc.date.accessioned | 2024-06-04T09:12:52Z | - |
dc.date.available | 2024-06-04T09:12:52Z | - |
dc.date.issued | 2023 | - |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | http://hdl.handle.net/10662/21386 | - |
dc.description.abstract | If a mechanism of more efficient glycolysis depending on pyruvate is present in stallion spermatozoa, detrimental effects of higher glucose concentrations that are common in current commercial extenders could be counteracted. To test this hypothesis, were incubated in a 67 mM Glucose modified Tyrode’s media in the presence of 1- or 10-mM pyruvate and in the Tyrode’s basal media which contains 5 mM glucose. incubated for 3 h at 37 ◦C in 67 mM Tyrode’s media with 10 mM pyruvate showed increased in comparison with aliquots incubated in Tyrode’s 5 mM glucose and Tyrode’s 67 mM glucose (57.1 ± 3.5 and 58.1 ± 1.9 to 73.0 ± 1.1 %; P < 0.01). Spermatozoa incubated in Tyrode’s with 67 mM glucose 10 mM pyruvate maintained the viability along the 64.03 ± 15.4 vs 61.3 ± 10.2), while spermatozoa incubated in 67 mM Glucose-Tyrode’s a decrease in viability (38.01 ± 11.2, P < 0.01). 40 mM oxamate, an inhibitor of the lactate dehydrogenase LDH, reduced sperm viability (P < 0.05, from 76 ± 5 in 67 mM Glucose/10 mM pyruvate to 68.0 ± 4.3 %, P < 0.05). Apoptotic markers increased in the presence of oxamate. (P < 0.01). UHPLC/MS/MS showed that 10 mM pyruvate pyruvate, lactate, ATP and NAD+ while phosphoenolpyruvate decreased. The that explain the improvement of in presence of 10 mM pyruvate involve the conversion of lactate to pyruvate and increased NAD+ enhancing the efficiency of the glycolysis. | es_ES |
dc.description.sponsorship | The authors received financial support for this study from the Ministerio de Ciencia-European Fund for Regional evelopment (EFRD), Madrid, Spain, grant PID2021-122351OB-I00. Junta de Extremadura- FEDER (IB 20008). GLGF holds a PhD grant from the Ministry of Science, Madrid, Spain (PRE2018-083354), LBR holds a PhD grant from the Ministry of Science, Madrid, Spain (PRE2022-103090). | es_ES |
dc.format.extent | 12 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | Attribution-NonCommercial 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Semental | es_ES |
dc.subject | Esperma | es_ES |
dc.subject | Piruvato | es_ES |
dc.subject | Análisis de semen asistido por ordenador (CASA) | es_ES |
dc.subject | Citometría de flujo | es_ES |
dc.subject | Metabolomics | es_ES |
dc.subject | Stallion | es_ES |
dc.subject | Sperm | es_ES |
dc.subject | Pyruvate | es_ES |
dc.subject | Computer Assisted Semen Analysis (CASA) | es_ES |
dc.subject | Flow cytometry | es_ES |
dc.subject | Metabolomics | es_ES |
dc.title | Pyruvate enhances stallion sperm function in high glucose media improving overall metabolic efficiency | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | openAccess | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3104.11 Reproducción | es_ES |
dc.subject.unesco | 2401.04 Citología Animal | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Martín Cano, F.E., Gaitskell Phillips, G., Becerro Rey, L., Álvarez, E. de S., Masot Gómez-Landero, A.J., Redondo García, E., Silva Rodríguez, A., Ortega Ferrusola, C., Gil Anaya, M.C., Peña Vega, F.J. (2023). Theriogenology, 215, 11.019, 1-12. https://doi.org/10.1016/j.theriogenology.2023.11.019 | es_ES |
dc.type.version | publishedVersion | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Medicina Animal | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Química Analítica | es_ES |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0093691X23004521?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.theriogenology.2023.11.019 | - |
dc.identifier.publicationtitle | Theriogenology | es_ES |
dc.identifier.publicationfirstpage | 1 | es_ES |
dc.identifier.publicationlastpage | 12 | es_ES |
dc.identifier.publicationvolume | 215 | - |
dc.identifier.e-issn | 1879-3231 | - |
dc.identifier.orcid | 0000-0003-4971-4883 | es_ES |
dc.identifier.orcid | 0000-0003-4308-7903 | es_ES |
dc.identifier.orcid | 0009-0007-6984-0760 | es_ES |
dc.identifier.orcid | 0009-0009-8939-9647 | es_ES |
dc.identifier.orcid | 0000-0003-0713-7061 | es_ES |
dc.identifier.orcid | 0000-0001-7702-5300 | es_ES |
dc.identifier.orcid | 0000-0002-7698-8735 | es_ES |
dc.identifier.orcid | 0000-0001-7041-9673 | es_ES |
Appears in Collections: | DMANI - Artículos |
Files in This Item:
File | Description | Size | Format | |
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j_theriogenology_2023_11_019.pdf | 7,66 MB | Adobe PDF | View/Open |
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