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http://hdl.handle.net/10662/20055
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Campo DC | Valor | idioma |
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dc.contributor.author | García Padilla, Carlos | - |
dc.contributor.author | García López, Virginio | - |
dc.contributor.author | Aranega Jiménez, Amelia | - |
dc.contributor.author | Franco Jaime, Diego | - |
dc.contributor.author | García Martínez, Virginio Enrique | - |
dc.contributor.author | López Sánchez, Carmen | - |
dc.date.accessioned | 2024-02-06T12:48:13Z | - |
dc.date.available | 2024-02-06T12:48:13Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://hdl.handle.net/10662/20055 | - |
dc.description.abstract | It is well known that multiple microRNAs play crucial roles in cardiovascular development, including miR-133a. Additionally, retinoic acid regulates atrial marker expression. In order to analyse the role of miR-133a as a modulator of retinoic acid signalling during the posterior segment of heart tube formation, we performed functional experiments with miR-133a and retinoic acid by means of microinjections into the posterior cardiac precursors of both primitive endocardial tubes in chick embryos. Subsequently, we subjected embryos to whole mount in situ hybridisation, immunohistochemistry and qPCR analysis. Our results demonstrate that miR-133a represses RhoA and Cdc42, as well as Raldh2/Aldh1a2, and the specific atrial markers Tbx5 and AMHC1, which play a key role during differentiation. Furthermore, we observed that miR-133a upregulates p21 and downregulates cyclin A by repressing RhoA and Cdc42, respectively, thus functioning as a cell proliferation inhibitor. Additionally, retinoic acid represses miR-133a, while it increases Raldh2, Tbx5 and AMHC1. Given that RhoA and Cdc42 are involved in Raldh2 expression and that they are modulated by miR-133a, which is influenced by retinoic acid signalling, our results suggest the presence of a negative feedback mechanism between miR-133a and retinoic acid during early development of the posterior cardiac tube segment. Despite additional unexplored factors being possible contributors to this negative feedback mechanism, miR-133a might also be considered as a potential therapeutic tool for the diagnosis, therapy and prognosis of cardiac diseases. | es_ES |
dc.description.sponsorship | This work has been financed with research grants IB18123 (to CL-S) and GR21174 (to VG-M, CTS005) from the Junta de Extremadura, with FEDER co-financing, and CTS-446 (to DF and AA) from the Junta de Andalucía Regional Council. | es_ES |
dc.format.extent | 15 p. | es_ES |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Attribution4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | MiR-133a | es_ES |
dc.subject | Atrial differentiation | es_ES |
dc.subject | Diferenciación auricular | es_ES |
dc.subject | Señalización de ácido retinoico | es_ES |
dc.subject | RhoA | es_ES |
dc.subject | Cdc42 | es_ES |
dc.subject | Raldh2 | es_ES |
dc.subject | Desarrollo cardíaco | es_ES |
dc.subject | Retinoic acid signalling | es_ES |
dc.subject | Cardiac development | es_ES |
dc.title | Inhibition of RhoA and Cdc42 by miR-133a modulates retinoic acid signalling during early development of posterior cardiac tube segment | es_ES |
dc.type | article | es_ES |
dc.description.version | peerReviewed | es_ES |
europeana.type | TEXT | en_US |
dc.rights.accessRights | openAccess | es_ES |
dc.subject.unesco | 2407 Biología Celular | es_ES |
europeana.dataProvider | Universidad de Extremadura. España | es_ES |
dc.identifier.bibliographicCitation | Garcia-Padilla C, Garcia-Lopez V, Aranega A, Franco D, Garcia-Martinez V, Lopez-Sanchez C. Inhibition of RhoA and Cdc42 by miR-133a Modulates Retinoic Acid Signalling during Early Development of Posterior Cardiac Tube Segment. Int J Mol Sci. 2022 Apr 10;23(8):4179 | es_ES |
dc.type.version | publisedVersion | es_ES |
dc.contributor.affiliation | Universidad de Jaén | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Departamento de Anatomía, Biología Celular y Zoología | es_ES |
dc.contributor.affiliation | Universidad de Extremadura. Instituto Universitario de Biomarcadores de Patologías Metabólicas | - |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/23/8/4179 | es_ES |
dc.identifier.doi | 10.3390/ijms23084179 | - |
dc.identifier.publicationtitle | International Journal of Molecular Science | es_ES |
dc.identifier.publicationissue | 8 | es_ES |
dc.identifier.publicationfirstpage | 4179-1 | es_ES |
dc.identifier.publicationlastpage | 4179-15 | es_ES |
dc.identifier.publicationvolume | 23 | es_ES |
dc.identifier.orcid | 0000-0003-1489-7296 | es_ES |
dc.identifier.orcid | 0000-0002-7760-1985 | es_ES |
dc.identifier.orcid | 0000-0003-3296-2071 | es_ES |
dc.identifier.orcid | 0000-0003-2061-3157 | - |
Colección: | DABCZ - Artículos |
Archivos
Archivo | Descripción | Tamaño | Formato | |
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ijms23084179.pdf | 2,7 MB | Adobe PDF | Descargar |
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